Ibuprofen administration attenuates serum TNF levels, hepatic glutathione depletion, hepatic apoptosis and mouse mortality after Fas stimulation ScienceDirect Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine Homocysteine May Decrease Glucose Uptake and Alter the Akt GSK3 GLUT1 Signaling Pathway in Hippocampal Slices: Neuroprotective Effects of Rivastigmine and Ibuprofen Molecular Neurobiology Springer Nature Link Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Acetaminophen Toxicity: How Genetic Susceptibility Combines with Environmental Factors